1982 Engineering durable root-knot nematode resistance in crops by RNAi silencing of a root-knot nematode parasitism gene
نویسندگان
چکیده
Secreted proteins coded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes. An essential parasitism gene, designated as 16D10, encodes a conserved root-knot nematode secretory peptide that stimulates root growth and functions as a ligand for a plant transcription factor. Plants were engineered to silence this parasitism gene in root-knot nematodes using RNA interference (RNAi). In planta expression of 16D10 dsRNA in Arabidopsis resulted in resistance against the four common root-knot nematode species (Meloidogyne spp.). No known natural resistance gene elicits this wide and effective range of root-knot nematode resistance. Genetically modifying crop cultivars to express dsRNA that silence essential nematode parasitism genes represents a viable and flexible means to develop novel durable broadspectrum root-knot nematode resistant crops. This strategy could also be used to develop root-knot nematode resistant crops for which natural resistance genes have not been found.
منابع مشابه
Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene.
Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. We used in vitro and in vivo RNA interference approaches to silence th...
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